It also contains an indicator that is blue when the jar contains oxygen and colourless when it's in anaerobic conditions.
The first procedure performed was an isolation of my unknown bacteria with the goal of obtaining a pure culture. It is mostly used for observing bacteria that are difficult to stain. The Identification of two unknown species of bacteria in tube 72 are Serratia marcescens and Micrococcus luteus References: The litmus milk test was performed on both Gram positive and Gram negative bacteria to differentiate bacteria based on various reactions that occur in skim milk supplemented with a litmus pH indicator.
An Oxidase test was performed on the Gram negative bacteria to determine if the bacteria are positive for cytochrome c oxidase.
This test is mostly used on streptococci species: The media used is peptone-iron agar. Biochemical properties continued Another test is whether or not your unknown has an hemolytic reaction. The Litmus milk test resulted in no change therefore ruling out all bacteria except for M.
Rinsing and drying surfaces after use can also prevent the establishment of the bacteria by removing its food source and making the environment less hospitable 2. The primary stain is malachite green which is heat-fixed followed by a counter stain with safranin.
It helps to slowly eliminate any bacteria that do not correspond with the results of certain tests. After the gram stain I reformed specific tests based of those results of the stain to determine what my unknown could be.
Some species make very particular colonies. You can also test for inulin fermentation. Bacillus subtilis creates a subterminal spore and Clostridium tetanomorphum has a terminal spore.
In other words, does it need oxygen to grow or can it use fermentation or anaerobic respiration. The litmus milk test was performed on both Gram positive and Gram negative bacteria to differentiate bacteria based on various reactions that occur in skim milk supplemented with a litmus pH indicator.
There was separate colony growth that where two different colors one red and one yellow on the streak plate incubated 25C. It's mostly a technique to rule out some options and to make sure we are dealing with a bacteria and not for example a mold.
The medium turns red if the test is positive for E. The following test is the coagulase test which shows if bacteria are capable of coagulating oxolated plasma. It is positive for E. It tests if the bacterium has the permease required to take up the citrate and use it as the sole carbon source.
Two slants where made with each colony growth and incubated for 48 hours 25C. Identifying Unknown Bacteria | Essay. Print Reference this. Disclaimer: while these two tests were not conducted by the students, they were still an important part of the identification process.
is usually performed before any biochemical tests “in order to adequately study and characterizeâ€¦.the bacterial species.” (Harley. Identification of Two Unknown Bacteria Abstract The purpose of this experiment was to learn how to isolate and identify unknown bacteria using methods from the lab.
Unknown 9 was used for this experiment. /5(3). There are two main types of bacteria, gram negative and gram-positive.
The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number The following are the tests that were used to identify the two different bacteria. Microbiology Unknown and Purpose of Microbiology Essay - Part 3. Microbiology Unknown #94 The purpose of this report was to identify an unknown bacterium by applying methods that were previously learned and conducted in microbiology lab class - Microbiology Unknown and Purpose of Microbiology Essay introduction.
Unknown Bacteria Lab Report Essay. isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor.
This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class.
Unknown Bacteria Lab Report Essay. isolate and identify two unknown bacteria from a mixed culture provided to us by our instructor. This study was done by applying all of the methods that have been instructed on thus far in microbiology laboratory class.Identifying two unknown species of bacteria essay